It is true the majority of the Wynwood Breweries have been sold to macro brews in an ironic twist, though some of them still seem to have a bit of autonomy. It was particularly sad to see WBC get bought by CBA (which is just a sub of InBev) a couple of years ago. But as long as the beer remains solid and evolving, I can't get too upset over it. Unfortunately, getting bought up by some of these beer giants does tend to lend itself towards a certain staleness and unwillingness to experiment.
I used to love The Room back in the day. Definitely a more European vibe of beers, as well as soon neat wines. Had my first lambic there. Was a good spot for a date. They are closed now, but they were open since at least 2003 (I know I took a couple of DGs to The Room while I was still at UM and I am a 2004 grad). Titanic as another early Miami staple with micro brews on tap is a good call and one that I am ashamed to have forgotten to include.
You are definitely right that Lokal opened before Kush and Union. I think Union is owned by the same people as Kush and maybe some of the same people as Lokal. I am pretty sure the original Filling Station was earlier though (their move near Funky Buddah was definitely later), since I vaguely remember Lokal opening around the time I was graduating from law school.
Union is a pretty cool spot, though. Only been there about twice in the last 4 years because it's a pain in the a$$ if you live in NW Dade, especially with so many solid options in Doral.